Anticorps Polyclonal de lapin anti-RTN2

• Phare
• Validé par KD/KO

RTN2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11168-1-AP

Synonymes

NSP like protein 1, NSP like protein I, NSP2, NSPL1, NSPLI, reticulon 2, RTN2

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 11168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11168-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11168-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11168-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11168-1-AP (RTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 11168-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11168-1-AP (RTN2 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 11168-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 11168-1-AP (RTN2 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

11168-1-AP cible RTN2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTN2 Protéine recombinante Ag1655
• Nom complet: reticulon 2
• Masse moléculaire calculée: 59 kDa, 51 kDa, 22 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC014244
• Symbole du gène: RTN2
• Identification du gène (NCBI): 6253
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN2 gene produces three isoforms termed RTN2-A, RTN2-B, and RTN2-C. Isoform RTN2-C has a calculated molecular weight of 22 kDa and is highly expressed in skeletal muscle.

Publications

Species	Application	Title
human	IHC	Mol Med Rep; RTN2, a new member of circadian clock genes identified by database mining and bioinformatics prediction, is highly expressed in ovarian cancer; Authors - Xiaojiao Zheng
human	WB,IHC	Cell Death Dis; Reticulon 2 promotes gastric cancer metastasis via activating endoplasmic reticulum Ca2+ efflux-mediated ERK signalling.; Authors - Shushu Song ; KD Validated
human	WB,IHC,IP	Cell Signal; O-GlcNAcylation enhances Reticulon 2 protein stability and its promotive effects on gastric cancer progression; Authors - Gaojia Wang
mouse	WB	Nature; Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy; Authors - Hector Foronda