Anticorps Polyclonal de lapin anti-RTN1 (Isoform RTN1-C)

• Phare
• Validé par KD/KO

RTN1 (Isoform RTN1-C) Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 15048-1-AP

Synonymes

NSP, NSP-C, reticulon 1, RTN1, RTN1 (Isoform RTN1-C), RTN1-C

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Galerie de données de validation

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  WB analysis using 15048-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15048-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15048-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 15048-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15048-1-AP (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu cérébral humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

15048-1-AP cible RTN1 (Isoform RTN1-C) dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTN1 (Isoform RTN1-C) Protéine recombinante Ag7031
• Nom complet: reticulon 1
• Masse moléculaire calculée: 84 kDa, 39 kDa, 24 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC000314
• Symbole du gène: RTN1-C
• Identification du gène (NCBI): 6252
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. Human RTN1 (also known as NSP) gene is expressed predominantly in neuroendocrine tissues, and produces three isoforms termed RTN1-A (NSP-A), RTN1-B (NSP-B), and RTN1-C (NSP-C). This antibody is raised against the full-length protein of human RTN1-C.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Combinatorial selective ER-phagy remodels the ER during neurogenesis; Authors - Melissa J Hoyer
mouse, rat	WB, IF	Cell Death Dis; RTN1-C mediates cerebral ischemia/reperfusion injury via ER stress and mitochondria-associated apoptosis pathways.; Authors - Lingli Gong ; KD Validated
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu
rat,mouse	WB	Acta Biochim Biophys Sin (Shanghai); RTN1-C mediates cerebral ischemia/reperfusion injury via modulating autophagy.; Authors - Jun Ling ; KD Validated
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu
mouse	WB	Brain Res Bull; RTN1-C is involved in high glucose-aggravated neuronal cell subjected to oxygen-glucose deprivation and reoxygenation injury via endoplasmic reticulum stress.; Authors - Muya Lin