Anticorps Polyclonal de lapin anti-RTEL1

RTEL1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 25337-1-AP

Synonymes

bK3184A7.3, C20orf41, KIAA1088, NHL, Novel helicase like, RTEL, RTEL1

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Galerie de données de validation

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  WB analysis of BGC-823 using 25337-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25337-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 25337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25337-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25337-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 25337-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25337-1-AP

  IP result of anti-RTEL1 (IP:25337-1-AP, 4ug; Detection:25337-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 25337-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RTEL1 antibody (25337-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules C6, cellules HeLa, cellules HepG2, cellules PC-3, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

25337-1-AP cible RTEL1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: regulator of telomere elongation helicase 1
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 133 kDa
• Numéro d’acquisition GenBank: NM_016434
• Symbole du gène: RTEL1
• Identification du gène (NCBI): 51750
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
zebrafish	WB	Nucleic Acids Res; Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.; Authors - Jingzhen Li
human	WB	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang ; KD Validated