Anticorps Polyclonal de lapin anti-RSPH9

RSPH9 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 23253-1-AP

Synonymes

C6orf206, CILD12, MRPS18AL1, RSPH9

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Galerie de données de validation

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  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 23253-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 23253-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23253-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23253-1-AP (RSPH9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules NIH/3T3, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

23253-1-AP cible RSPH9 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSPH9 Protéine recombinante Ag19745
• Nom complet: radial spoke head 9 homolog (Chlamydomonas)
• Masse moléculaire calculée: 276 aa, 31 kDa
• Poids moléculaire observé: 31-35 kDa
• Numéro d’acquisition GenBank: BC029519
• Symbole du gène: RSPH9
• Identification du gène (NCBI): 221421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB, IF	Hum Mol Genet; Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.; Authors - Jintao Zhang
		Eur J Hum Genet; Bi-allelic variants in human TCTE1/DRC5 cause asthenospermia and male infertility.; Authors - Shushu Zhou
mouse	WB,IF	Cell Rep; Differential requirements of IQUB for the assembly of radial spoke 1 and the motility of mouse cilia and flagella; Authors - Xin Zhang
mouse	WB	EBioMedicine; CFAP70 is a solid and valuable target for the genetic diagnosis of oligo-astheno-teratozoospermia in infertile men; Authors - Hui-Juan Jin
human	IF	Cell; Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility; Authors - Lunni Zhou
mouse	WB	Elife; LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility; Authors - Jae Yeon Hwang