Anticorps Polyclonal de lapin anti-RRM2

RRM2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(24)

N° de cat : 11661-1-AP

Synonymes

EC:1.17.4.1, R2, RR2, RR2M

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Galerie de données de validation

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  WB analysis using 11661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11661-1-AP (RRM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11661-1-AP

  IP result of anti-RRM2 (IP:11661-1-AP, 4ug; Detection:11661-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human appendicitis using 11661-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11661-1-AP (RRM2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 11661-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11661-1-AP (RRM2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 11661-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11661-1-AP (RRM2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11661-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using RRM2 antibody (11661-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11661-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RRM2 (11661-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HUVEC, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu d'appendicite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 17 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

11661-1-AP cible RRM2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RRM2 Protéine recombinante Ag2203
• Nom complet: ribonucleotide reductase M2 polypeptide
• Masse moléculaire calculée: 389 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC030154
• Symbole du gène: RRM2
• Identification du gène (NCBI): 6241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleotide reductase M2 subunit is one of two subunits that constitute ribonucleotide reductase, the enzyme that catalyzes the conversion of ribonucleotide 5'-diphosphates into 2'-deoxyribonucleotides, a rate-limiting step in the production of 2'-deoxyribonucleoside 5'-diphosphates (dNTP) required for DNA synthesis and repair that is required for DNA synthesis and repair [PMID:20825972, 19250552]. RRM2 is only expressed during the late G1/early S phase, and degraded in late S phase, and the activity of RNR, and therefore DNA synthesis and cell proliferation, is controlled during the cell cycle by the synthesis and degradation of RRM2 subunit [PMID:3894352].

Publications

Species	Application	Title
mouse	IF	Cell Rep; The mechano-chemical circuit in fibroblasts and dendritic cells drives basal cell proliferation in psoriasis; Authors - Jingwei Jiang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang
human	IHC	Front Cell Dev Biol; A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.; Authors - Zhi-Bin Ke
	WB	Antiviral Res; Long-term HIV-1 infection induces an antiviral state in primary macrophages.; Authors - Maria Pujantell
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
human	WB	Biomed Pharmacother; Identification of osalmid metabolic profile and active metabolites with anti-tumor activity in human hepatocellular carcinoma cells.; Authors - Zhe Wu

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