Anticorps Polyclonal de lapin anti-RREB1-Specific

• Phare
• Validé par KD/KO

RREB1-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20280-1-AP

Synonymes

FINB, HNT, LZ321, RREB 1, RREB1, RREB1-Specific, Zep 1

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Galerie de données de validation

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  WB analysis using 20280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 20280-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20280-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20280-1-AP (RREB1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human urothelial carcinoma using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20280-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20280-1-AP (RREB1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de carcinome urothélial humain, tissu de cancer du pancréas humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20280-1-AP cible RREB1-Specific dans les applications de WB, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ras responsive element binding protein 1
• Masse moléculaire calculée: 181 kDa
• Poids moléculaire observé: 160-181 kDa, 250 kDa
• Numéro d’acquisition GenBank: NM_001003698
• Symbole du gène: RREB1
• Identification du gène (NCBI): 6239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RREB1, also named as FINB and Zep-1, belongs to the krueppel C2H2-type zinc-finger protein family. RREB1 is a transcription factor that binds specifically to the RAS-responsive elements (RRE) of gene promoters. It may be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression. RREB1 represses the angiotensinogen gene. RREB1 can induce expression of p53 and represse p16 which associated with the expression of tumour. It has been reported that the RREB1 can be a candidate gene for type 2 diabetes associated end-stage kidney disease. To some extent, the decrease of zinc in human pancreatic adenocarcinoma is associated with the downregulation of RREB1. RREB1 has some isoforms with 250 kDa, 180-200 kDa and 140-160 kDa protein.(PMID: 21703425, 15067362, 25050557, 25027322)

Publications

Species	Application	Title
human	WB,IHC	Cell Cycle; Knockdown of RREB1 inhibits cell proliferation via enhanced p16 expression in gastric cancer.; Authors - Qi Gao ; KD Validated
human	ChIP,WB	Cell Rep; Oncogenic KRAS effector USP13 promotes metastasis in non-small cell lung cancer through deubiquitinating β-catenin; Authors - Yanguan Guo ; KD Validated