Anticorps Polyclonal de lapin anti-RSK2

• Phare
• Validé par KD/KO

RSK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 23762-1-AP

Synonymes

RPS6KA3, ISPK1, ISPK 1, Insulin-stimulated protein kinase 1, HU 3

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Galerie de données de validation

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  WB analysis of K-562 using 23762-1-AP

  WB result of RSK2 antibody (23762-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RSK2 transfected K-562 cells.

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  WB analysis using 23762-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 23762-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 23762-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23762-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23762-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23762-1-AP (RSK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:4000) with K-562 cells lysate 3680 ug.

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  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000ug.

• 

  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug.

• 

  IP experiment of K-562 using 23762-1-AP

  IP result of anti-RSK2 (IP:23762-1-AP, 4ug; Detection:23762-1-AP 1:1000) with K-562 cells lysate 4000 ug.

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  IHC staining of human kidney using 23762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human kidney using 23762-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23762-1-AP (RSK2 antibody at dilution of 1:50(under 40x lens).

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  IF Staining of mouse brain using 23762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 23762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23762-1-AP (RSK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 23762-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 23762-1-AP (RSK2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HUVEC using 23762-1-AP

  Immunofluorescent analysis of HUVEC cells using 23762-1-AP (RSK2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules HepG2, cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

23762-1-AP cible RSK2 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RSK2 Protéine recombinante Ag20620
• Nom complet: ribosomal protein S6 kinase, 90kDa, polypeptide 3
• Masse moléculaire calculée: 740 aa, 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC096301
• Symbole du gène: RSK2
• Identification du gène (NCBI): 6197
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS6KA3(Ribosomal protein S6 kinase alpha-3) is also named as ISPK1, MAPKAPK1B, RSK2 and belongs to the protein kinase superfamily. It localizes at the kinetochores under spindle checkpoint conditions, and during mitosis, associated with the centrosomes, the spindle and at the mid-body(PMID:20383198). It is required for EGF activated phosphorylation of histone H3, which may cause chromatin remodeling and facilitate gene transcription and maybe playing an essential regulatory role in protein synthesis during cell proliferation.

Publications

Species	Application	Title
		Theranostics; S6K1 phosphorylation-dependent degradation of Mxi1 by β-Trcp ubiquitin ligase promotes Myc activation and radioresistance in lung cancer.; Authors - Yumei Huang
human	WB	Cell Death Differ; β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.; Authors - Jia Ma
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Int J Biochem Cell Biol; DHPAC, a novel synthetic microtubule destabilizing agent, possess high anti-tumor activity in vincristine-resistant oral epidermoid carcinoma in vitro and in vivo.; Authors - Ying Zhang
human	WB	Prostate; MicroRNA181c inhibits prostate cancer cell growth and invasion by targeting multiple ERK signaling pathway components.; Authors - Zhengzheng Su
human	WB	PLoS One; A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction; Authors - Junwei Pan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (09-04-2020)

Very clean bands of RSK2 in immunoblotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer cells