Anticorps Polyclonal de lapin anti-RPS15

RPS15 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 14957-1-AP

Synonymes

40S ribosomal protein S15, ribosomal protein S15, RIG, RIG protein, RPS15

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Galerie de données de validation

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  WB analysis of human liver using 14957-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14957-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14957-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14957-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14957-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14957-1-AP (RPS15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14957-1-AP (RPS15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14957-1-AP (RPS15 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14957-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14957-1-AP (RPS15 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14957-1-AP

  Immunofluorescent analysis of Hela cells, using RPS15 antibody 14957-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HeLa, cellules L02, tissu cérébral de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer de l'estomac humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu hépatique de rat, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

14957-1-AP cible RPS15 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS15 Protéine recombinante Ag6795
• Nom complet: ribosomal protein S15
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC064908
• Symbole du gène: RPS15
• Identification du gène (NCBI): 6209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.

Publications

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	IF	Cell Prolif; An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms; Authors - Nan Zhang
human	WB	Elife; The molecular basis of coupling between poly(A)-tail length and translational efficiency.; Authors - Kehui Xiang
human		Cell Rep; FKBP10 Regulates Protein Translation to Sustain Lung Cancer Growth.; Authors - Giorgio Ramadori
human	WB	Elife; The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.; Authors - Diego Acosta-Alvear
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Advait (Verified Customer) (02-22-2019)

4% PFA fixationBlocking buffer: BSA with 0.05% SaponinIncubate at 4 degrees overnight for best staining quality.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa

RPS15 Antibody Western Blot,Immunofluorescence, validation (1:100 dilution) in HeLa (Cat no:14957-1-AP)