Anticorps Polyclonal de lapin anti-RPL31

• Phare
• Validé par KD/KO

RPL31 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 16497-1-AP

Synonymes

ribosomal protein L31, Large ribosomal subunit protein eL31, 60S ribosomal protein L31

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Galerie de données de validation

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  WB analysis of HepG2 using 16497-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16497-1-AP

  WB result of RPL31 antibody (16497-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL31 transfected HepG2 cells.

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  WB analysis of NIH/3T3 using 16497-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16497-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16497-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16497-1-AP (RPL31 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16497-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16497-1-AP (RPL31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

16497-1-AP cible RPL31 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL31 Protéine recombinante Ag9648
• Nom complet: ribosomal protein L31
• Masse moléculaire calculée: 125 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC017343
• Symbole du gène: RPL31
• Identification du gène (NCBI): 6160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center [PMID:22714919].

Publications

Species	Application	Title
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (01-30-2020)

Weak band around 15kDa

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart