Anticorps Polyclonal de lapin anti-RPL29

RPL29 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 15799-1-AP

Synonymes

60S ribosomal protein L29, HIP, HUMRPL29, ribosomal protein L29, RPL29

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Galerie de données de validation

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  WB analysis using 15799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15799-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 15799-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15799-1-AP (RPL29 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 15799-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL29 antibody (15799-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293T, cellules HepG2, cellules HUVEC, cellules NIH/3T3, tissu pancréatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

15799-1-AP cible RPL29 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL29 Protéine recombinante Ag8510
• Nom complet: ribosomal protein L29
• Masse moléculaire calculée: 159 aa, 18 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC008926
• Symbole du gène: RPL29
• Identification du gène (NCBI): 6159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.

Publications

Species	Application	Title
human	WB	Nature; Systematic quantitative analysis of ribosome inventory during nutrient stress.; Authors - Heeseon An
mouse	WB	J Cell Sci; Germline Deletion of Huntingtin Causes Male Infertility and Arrested Spermiogenesis in Mice.; Authors - Jinting Yan
human,mouse	WB	Sci Rep; The tissue specific regulation of miR22 expression in the lung and brain by ribosomal protein L29.; Authors - Mohammad Ishaque Ali
human	WB	Sci Adv; Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells; Authors - Chandreyee Datta

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sun (Verified Customer) (01-05-2023)

A great antibody with strong signal and specificity. We confirmed this antibody in HCT116 and HEK293T cell lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

FH

H (Verified Customer) (04-05-2020)

This antibody works very well. I used this antibody when screening clones for CRISPR knock-in.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPL29 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:15799-1-AP)