Anticorps Polyclonal de lapin anti-RPL26

• Phare
• Validé par KD/KO

RPL26 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 17619-1-AP

Synonymes

60S ribosomal protein L26, ribosomal protein L26, RPL26

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Galerie de données de validation

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  WB analysis of K-562 using 17619-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17619-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17619-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17619-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17619-1-AP (RPL26 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17619-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RPL26 antibody (17619-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules HepG2, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17619-1-AP cible RPL26 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL26 Protéine recombinante Ag11821
• Nom complet: ribosomal protein L26
• Masse moléculaire calculée: 145 aa, 17 kDa
• Poids moléculaire observé: 17-22 kDa
• Numéro d’acquisition GenBank: BC071664
• Symbole du gène: RPL26
• Identification du gène (NCBI): 6154
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	Genes (Basel); Transcriptional Regulation of CD40 Expression by 4 Ribosomal Proteins via a Functional SNP on a Disease-Associated CD40 Locus.; Authors - Meijuan Zou ; KD Validated
human	WB	Science; Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function; Authors - Wonyul Jang
human	WB	Brain; GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation; Authors - Yu Fan
human	WB,IHC,CoIP	Am J Cancer Res; UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc; Authors - Dalian Gong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (07-09-2020)

This antibody works well. I did crispr knock-in of RPL26 and it was easily detected by this antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HCT116

RPL26 Antibody Western Blot validation (1:2000 dilution) in HCT116 (Cat no:17619-1-AP)