Anticorps Polyclonal de lapin anti-RPL23

• Phare
• Validé par KD/KO

RPL23 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 16086-1-AP

Synonymes

60S ribosomal protein L17, 60S ribosomal protein L23, ribosomal protein L23, rpL17, RPL23

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Galerie de données de validation

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  WB analysis using 16086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16086-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16086-1-AP (RPL23 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16086-1-AP

  IP result of anti-RPL23 (IP:16086-1-AP, 4ug; Detection:16086-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16086-1-AP (RPL23 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16086-1-AP (RPL23 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16086-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16086-1-AP (RPL23 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules Jurkat, cellules NIH/3T3, cellules PC-3
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

16086-1-AP cible RPL23 dans les applications de WB, IP, IF, RIP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL23 Protéine recombinante Ag9120
• Nom complet: ribosomal protein L23
• Masse moléculaire calculée: 140 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC010114
• Symbole du gène: RPL23
• Identification du gène (NCBI): 9349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins [PMID:9582194]. RPL23 is a component of the 60 S large ribosomal subunit that interacts functionally with murine double minute 2 protein (MDM2 or HDM2 in humans). Upon binding to MDM2, RPL23 inhibits MDM2-mediated p53 degradation, thus activating p53 and inducing cellular apoptosis [PMID:15314174]. Intriguingly, RPL23 has also been suggested to be a negative regulator of apoptosis by suppressing the Miz1-induced transcription of the cell cycle inhibitors p15INK4B and p21CIP1[PMID:19160485].

Publications

Species	Application	Title
human	WB	Nat Cell Biol; LIN28 phosphorylation by MAPK/ERK couples signalling to the post-transcriptional control of pluripotency.; Authors - Kaloyan M Tsanov
human,mouse	IHC	EMBO J; Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.; Authors - Harrison Tudor Evans
human	WB	Blood; Defective ribosome assembly in Shwachman-Diamond syndrome.; Authors - Wong Chi C CC
mouse	WB,IHC	Sci Rep; Ribosomal protein L23 negatively regulates cellular apoptosis via the RPL23/Miz-1/c-Myc circuit in higher-risk myelodysplastic syndrome.; Authors - Yuekun Qi ; KD Validated
human	WB,IHC,IF,RIP	Front Oncol; Ribosomal Protein L23 Drives the Metastasis of Hepatocellular Carcinoma via Upregulating MMP9.; Authors - Minli Yang ; KD Validated
mouse		J Alzheimers Dis; Tobacco Smoke Exposure Impairs Brain Insulin/IGF Signaling: Potential Co-Factor Role in Neurodegeneration; Authors - Chetram Deochand