Anticorps Polyclonal de lapin anti-RPL15

RPL15 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16740-1-AP

Synonymes

60S ribosomal protein L15, EC45, ribosomal protein L15, RPL10, RPL15, RPLY10, RPYL10

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Galerie de données de validation

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  WB analysis of COLO 320 using 16740-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 16740-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16740-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16740-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 16740-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16740-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16740-1-AP (RPL15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules K-562, cellules SGC-7901, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

16740-1-AP cible RPL15 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL15 Protéine recombinante Ag9948
• Nom complet: ribosomal protein L15
• Masse moléculaire calculée: 204 aa, 24 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC070328
• Symbole du gène: RPL15
• Identification du gène (NCBI): 6138
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
pig	WB	Front Immunol; Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.; Authors - Xu Ye
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao
human	IF	Cell Rep; Senescent cells form nuclear foci that contain the 26S proteasome; Authors - Tomohiro Iriki
human	WB	Cell Rep; ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells; Authors - M Carmen Lafita-Navarro