Anticorps Polyclonal de lapin anti-RPL10

RPL10 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17013-1-AP

Synonymes

60S ribosomal protein L10, DXS648, DXS648E, Laminin receptor homolog, NOV, Protein QM, QM, ribosomal protein L10, RPL10, Tumor suppressor QM

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Galerie de données de validation

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  WB analysis using 17013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17013-1-AP (RPL10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17013-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17013-1-AP (RPL10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

17013-1-AP cible RPL10 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPL10 Protéine recombinante Ag9969
• Nom complet: ribosomal protein L10
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC003358
• Symbole du gène: RPL10
• Identification du gène (NCBI): 6134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPL10, also named as Large ribosomal subunit protein uL16, is a 214 amino acid protein, which belongs to the universal ribosomal protein uL16 family. RPL10 is a component of the large ribosomal subunit and is down-regulated during adipocyte, kidney, and heart differentiation. RPL10 shares 98% amino acid sequence with RPL10L, so RPL10 antibody is able to recognize RPL10 and RPL10L protein.

Publications

Species	Application	Title
human	WB	Cell Discov; Ribosomal RNA regulates chromosome clustering during mitosis.; Authors - Kai Ma
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
mouse	IF	Development; Lin28-mediated promotion of protein synthesis is critical for neural progenitor cell maintenance and brain development in mice.; Authors - Stephanie Herrlinger
human	WB	Brain; GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation; Authors - Yu Fan
human	WB	Cardiovasc Res; The translational landscape of human vascular smooth muscle cells identifies novel short ORF-encoded peptide regulators for phenotype alteration; Authors - Kang Li