Anticorps Polyclonal de lapin anti-RPGRIP1

• Phare
• Validé par KD/KO

RPGRIP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13214-1-AP

Synonymes

CORD9, LCA6, RGI1, RGRIP, RPGR interacting protein 1, RPGRIP, RPGRIP1, RPGRIP1d

---

Galerie de données de validation

• 

  WB analysis using 13214-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13214-1-AP (RPGRIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 13214-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13214-1-AP (RPGRIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules Y79, cellules HEK-293, cellules HL-60
• Résultats positifs en IHC: tissu oculaire de souris, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13214-1-AP cible RPGRIP1 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPGRIP1 Protéine recombinante Ag4089
• Nom complet: retinitis pigmentosa GTPase regulator interacting protein 1
• Masse moléculaire calculée: 147 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039089
• Symbole du gène: RPGRIP1
• Identification du gène (NCBI): 57096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPGRIP1 is a photoreceptor protein that interacts with retinitis pigmentosa GTPase regulator protein and is a key component of cone and rod photoreceptor cells. The biological functions of RPGRIP1 are complex. Mutations in this gene lead to autosomal recessive congenital blindness. In the eye, it is expressed in amacrine neurons and photoreceptors, and in numerous other tissues, albeit at greatly reduced levels. Moreover, it exists multiple isoforms with species-specific subcellular localization patterns, and the different isoforms perform cell-specific functions. Within photoreceptors, RPGRIP1 localizes predominantly to outer segments (OS) of rods and cones in humans, whereas in bovine eyes, it is found only in rod OS. In contrast, in mice, RPGRIP1 is found primarily in connecting cilia (CC), where it localizes between the ciliary axoneme and the plasma membrane. RPGRIP1 has multiple isoforms with MW 147 kDa, 70-76 kDa, 103 kDa, and 66 kDa.

Publications

Species	Application	Title
mouse	WB,IF	EMBO J; Cell type-specific regulation of ciliary transition zone assembly in vertebrates.; Authors - Antonia Wiegering ; KO Validated