Anticorps Polyclonal de lapin anti-RPAP2

• Phare
• Validé par KD/KO

RPAP2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 17401-1-AP

Synonymes

C1orf82, RPAP2

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Galerie de données de validation

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  WB analysis using 17401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17401-1-AP (RPAP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17401-1-AP (RPAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17401-1-AP

  IP result of anti-RPAP2 (IP:17401-1-AP, 4ug; Detection:17401-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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  IHC staining of human kidney using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 17401-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RPAP2 antibody (17401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules MOLT-4, cellules THP-1
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

17401-1-AP cible RPAP2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPAP2 Protéine recombinante Ag11374
• Nom complet: RNA polymerase II associated protein 2
• Masse moléculaire calculée: 612 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039014
• Symbole du gène: RPAP2
• Identification du gène (NCBI): 79871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPAP2 belongs to the RNA Pol II-associated protein (RPAP) family, a set of proteins involved in human Pol II machinery. Recently it has been reported that RPAP2 is required for efficient transcription of snRNA genes by Pol II. siRNA-mediated knockdown of RPAP2 caused defects in snRNA gene expression. (22137580)

Publications

Species	Application	Title
human	WB	Mol Cell; Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes.; Authors - Egloff Sylvain S
human	WB	Mol Cell; Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate.; Authors - Tsun-Kai Chang ; KD Validated
human	WB	Nat Struct Mol Biol; RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.; Authors - Zuyao Ni ; KD Validated
human	WB	Nucleic Acids Res; Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.; Authors - Forget Diane D
human	WB	PLoS One; Herpes Simplex Virus 1 (HSV-1) ICP22 Protein Directly Interacts with Cyclin-Dependent Kinase (CDK)9 to Inhibit RNA Polymerase II Transcription Elongation.; Authors - Justyna Zaborowska
human	WB, IF	Histopathology; Abnormal expression of RNA polymerase II associated proteins in muscle of patients with myofibrillar myopathies.; Authors - Valeria Guglielmi