Anticorps Polyclonal de lapin anti-RPA2

RPA2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 10412-1-AP

Synonymes

REPA2, Replication factor A protein 2, replication protein A2, 32kDa, RF A protein 2, RP A p32, RP A p34, RPA2, RPA32, RPA34

---

Galerie de données de validation

• 

  WB analysis using 10412-1-Ap

  Various lysates were subjected to SDS PAGE followed by western blot with 10412-1-Ap (RPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10412-1-Ap

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10412-1-AP (RPA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10412-1-Ap

  IP result of anti-RPA2 (IP:10412-1-Ap, 4ug; Detection:10412-1-Ap 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 10412-1-Ap

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10412-1-Ap (RPA2 antibody) at dilution of 1:100 (under 40x lens)..

• 

  IHC staining of human gliomas using 10412-1-Ap

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10412-1-Ap (RPA2 antibody) at dilution of 1:100 (under 10x lens)..

• 

  IF Staining of A431 using 10412-1-Ap

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (10412-1-Ap) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

10412-1-AP cible RPA2 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPA2 Protéine recombinante Ag0690
• Nom complet: replication protein A2, 32kDa
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC001630
• Symbole du gène: RPA2
• Identification du gène (NCBI): 6118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao ; KD Validated
human	WB	Mol Cell; C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.; Authors - Yongtai Bai
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB,IF,IP	Cancer Lett; Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.; Authors - Shuai Ma
human	WB	J Virol; Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression; Authors - Yanqun Zhao