Anticorps Polyclonal de lapin anti-RPA1

RPA1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 12448-1-AP

Synonymes

HSSB, MST075, REPA1, Replication factor A protein 1, Replication protein A 70 kDa DNA-binding subunit

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Galerie de données de validation

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  WB analysis using 12448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12448-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12448-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis using 12448-1-AP

  K-562 cell lysates were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12448-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12448-1-AP (RPA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules K-562, cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12448-1-AP cible RPA1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPA1 Protéine recombinante Ag3067
• Nom complet: replication protein A1, 70kDa
• Masse moléculaire calculée: 616 aa, 68 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC018126
• Symbole du gène: RPA1
• Identification du gène (NCBI): 6117
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Replication protein A 1 (RPA1) is a subunit of a stable heterotrimer replication protein A (RPA), which is conserved in almost all eukaryotic species (PMID: 27151292). Usually, RPA1, RPA2 and RPA3 function as a whole to participate in DNA replication, telomere maintenance, DNA recombination, DNA repair, and activation of DNA damage checkpoints. In the cell, RPA has a higher affinity to bind single strand DNA and then to form a fork protection complex with other components such as PTEN and OTUB1, thus maintains genome stability during replication(PMID: 26403191; PMID: 18469000).

Publications

Species	Application	Title
human	WB	Front Oncol; PMID: 31249811; Authors - Ma-Sha Huang

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