Anticorps Polyclonal de lapin anti-Kazrin

• Phare
• Validé par KD/KO

Kazrin Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11572-1-AP

Synonymes

KAZ, kazrin, KIAA1026, RP1 21O18.1, RP1-21O18.1

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Galerie de données de validation

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  WB analysis using 11572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11572-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis using 11572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (Kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11572-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11572-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11572-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11572-1-AP (kazrin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse skin using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris, tissu cutané de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cutané de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11572-1-AP cible Kazrin dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Kazrin Protéine recombinante Ag2152
• Nom complet: kazrin
• Masse moléculaire calculée: 415 aa, 46 kDa
• Poids moléculaire observé: 46 kDa, 37 kDa
• Numéro d’acquisition GenBank: BC035501
• Symbole du gène: Kazrin
• Identification du gène (NCBI): 23254
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin exists three isoforms:kazrin A (46.7 kDa), kazrin B (46 kDa), and kazrin C (37 kDa).

Publications

Species	Application	Title
mouse	WB,IF	J Invest Dermatol; Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.; Authors - Chhatriwala Mariya K MK ; KD Validated