Anticorps Polyclonal de lapin anti-RORB

RORB Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 17635-1-AP

Synonymes

NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB

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Galerie de données de validation

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  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17635-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17635-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17635-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17635-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17635-1-AP

  IP result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:300) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below

Informations sur le produit

17635-1-AP cible RORB dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RORB Protéine recombinante Ag11910
• Nom complet: RAR-related orphan receptor B
• Masse moléculaire calculée: 459 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC051830
• Symbole du gène: RORB
• Identification du gène (NCBI): 6096
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

Publications

Species	Application	Title
mouse	IF	Neuron; Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.; Authors - Chao Wang
mouse	IF	PLoS Biol; An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold; Authors - Wenny Wong
mouse	IF	eNeuro; Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.; Authors - Natalia Galindo-Riera

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Etienne (Verified Customer) (12-19-2022)

Did not work at all in human brain organoids (where the target is present)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoids

RORB Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:17635-1-AP)