Anticorps Polyclonal de lapin anti-ROGDI

• Phare
• Validé par KD/KO

ROGDI Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 17047-1-AP

Synonymes

Protein rogdi homolog, ROGDI, rogdi homolog (Drosophila)

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Galerie de données de validation

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  WB analysis of human brain using 17047-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17047-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17047-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17047-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17047-1-AP (ROGDI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17047-1-AP

  IP result of anti-ROGDI (IP:17047-1-AP, 4ug; Detection:17047-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17047-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17047-1-AP (ROGDI antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de souris, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, tissu placentaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

17047-1-AP cible ROGDI dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROGDI Protéine recombinante Ag10688
• Nom complet: rogdi homolog (Drosophila)
• Masse moléculaire calculée: 287 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC012901
• Symbole du gène: ROGDI
• Identification du gène (NCBI): 79641
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROGDI encoded by the ROGDI gene, which encodes a leucine-zipper protein with high expression in the human spinal cord and brain, acts as a positive regulator of cell proliferation. Defections of ROGDI are the cause of Kohlschuetter-Toenz syndrome (KTZS)

Publications

Species	Application	Title
human	IF	Am J Hum Genet; Mutations in ROGDI Cause Kohlschütter-T?nz Syndrome.; Authors - Schossig Anna A
Drosophila	IF	Am J Hum Genet; A nonsense mutation in the human homolog of Drosophila rogdi causes Kohlschutter-Tonz syndrome.; Authors - Mory Adi A
rat	WB, IF	Sci Rep; The Kohlschütter-Tönz syndrome associated gene Rogdi encodes a novel presynaptic protein.; Authors - Donatus Riemann
human	WB	Cancer Biol Ther; Downregulation of a novel human gene, ROGDI, increases radiosensitivity in cervical cancer cells.; Authors - Yi-Fan Chen ; KD Validated
mouse	IHC	Sci Rep; The Rogdi knockout mouse is a model for Kohlschütter-Tönz syndrome; Authors - Alexandra Jimenez-Armijo