Anticorps Polyclonal de lapin anti-ROD1

• Phare
• Validé par KD/KO

ROD1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 14027-1-AP

Synonymes

PTBP3, Regulator of differentiation 1, ROD1

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Galerie de données de validation

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  WB analysis of HL-60 using 14027-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14027-1-AP

  WB result of ROD1 antibody (14027-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROD1 transfected HeLa cells.

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  WB analysis of mouse thymus using 14027-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 14027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lymphoma using 14027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14027-1-AP cible ROD1 dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROD1 Protéine recombinante Ag5172
• Nom complet: ROD1 regulator of differentiation 1 (S. pombe)
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 55-59 kDa
• Numéro d’acquisition GenBank: BC044585
• Symbole du gène: ROD1
• Identification du gène (NCBI): 9991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROD1 is a RNA-binding protein that mediates pre-mRNA alternative splicing regulation, it plays a role in the regulation of cell proliferation, differentiation and migration. ROD1 exist some isoforms and also can be modified with the molecular weight range from 55-59 kDa.

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Res; The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.; Authors - Juan Chen ; KO Validated
		iScience; Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis; Authors - Qianqing Wang
human	WB	Cancer Cell Int; PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.; Authors - Yingji Chen
human	WB,IHC	Cancer Cell Int; PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway.; Authors - Qiong Wu
human	WB	Oncol Lett; Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus.; Authors - Ya Zhou
mouse	WB	Mol Cell; PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells; Authors - Camilla Iannone

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yaroslav (Verified Customer) (08-03-2022)

multiple bands detected at all tested dulitions (1:3000-1:10000)

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse embryonic stem cells

ROD1 Antibody Western Blot validation (1:5000 dilution) in mouse embryonic stem cells (Cat no:14027-1-AP)