Anticorps Polyclonal de lapin anti-ROCK2(middle)

• Phare
• Validé par KD/KO

ROCK2(middle) Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 21645-1-AP

Synonymes

KIAA0619, p164 ROCK 2, Rho kinase 2, ROCK2

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Galerie de données de validation

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  WB analysis of HeLa using 21645-1-AP

  WB result of ROCK2(middle) antibody (21645-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.

• 

  WB analysis of HT-1080 using 21645-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21645-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21645-1-AP

  WB result of ROCK2(middle) antibody (21645-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.

• 

  WB analysis of mouse brain using 21645-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21645-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21645-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21645-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21645-1-AP

  IP result of anti-ROCK2(middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 21645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21645-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 21645-1-AP

  Immunofluorescent analysis of Hela cells, using ROCK2 antibody 21645-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HeLa, cellules L02, cellules MCF-7, tissu cérébral de souris, tissu hépatique de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

21645-1-AP cible ROCK2(middle) dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ROCK2(middle) Protéine recombinante Ag16330
• Nom complet: Rho-associated, coiled-coil containing protein kinase 2
• Masse moléculaire calculée: 77 kDa, 161 kDa
• Poids moléculaire observé: 161 kDa
• Numéro d’acquisition GenBank: BC111801
• Symbole du gène: ROCK2
• Identification du gène (NCBI): 9475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).

Publications

Species	Application	Title
human	IHC	Oncotarget; Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.; Authors - Rachel Hiu Ha Ching
human	WB	Cells; The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.; Authors - Kathryn N Becker
rat	WB	Front Pharmacol; Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.; Authors - Penghui Yuan
mouse	WB	Mol Pharmacol; Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression.; Authors - Yanhua Wang
rat	WB	J Sex Med; FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.; Authors - Kai Cui
rat	WB,IHC	Andrology; JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.; Authors - K Liu