Anticorps Polyclonal de lapin anti-RNPS1

• Phare
• Validé par KD/KO

RNPS1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10555-1-AP

Synonymes

E5.1, LDC2, RNPS1, SR related protein LDC2

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Galerie de données de validation

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  WB analysis of HEK-293 using 10555-1-AP

  WB result of RNPS1 antibody (10555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNPS1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 10555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10555-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10555-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10555-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10555-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10555-1-AP

  IP result of anti-RNPS1 (IP:10555-1-AP, 4ug; Detection:10555-1-AP 1:800) with HEK-293 cells lysate 3200ug.

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  IF Staining of HEK-293 using 10555-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10555-1-AP (RNPS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Raji, tissu cérébral de souris, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

10555-1-AP cible RNPS1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNPS1 Protéine recombinante Ag0844
• Nom complet: RNA binding protein S1, serine-rich domain
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC001838
• Symbole du gène: RNPS1
• Identification du gène (NCBI): 10921
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro

Publications

Species	Application	Title
human	WB,IP	Mol Cell; AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.; Authors - Hana Cho
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
mouse	WB,IF	Biochem Biophys Res Commun; RNPS1 inhibition aggravates ischemic brain injury and promotes neuronal death.; Authors - Zhi Zhang ; KD Validated
human	WB,IHC,IF,IP	Int J Oral Sci; RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac4C modification; Authors - Xiaochen Wang ; KD Validated