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Anticorps Polyclonal de lapin anti-RNF212

RNF212 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 21178-1-AP

Synonymes

ring finger protein 212, RNF212, ZHP3

Galerie de données de validation

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  • Western Blot (WB) analysis of human heart tissue using RNF212 Polyclonal antibody (21178-1-AP)

    WB analysis of human heart using 21178-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21178-1-AP (RNF212 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBtissu cardiaque humain

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

21178-1-AP cible RNF212 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RNF212 Protéine recombinante Ag15457
Nom complet ring finger protein 212
Masse moléculaire calculée 297 aa, 33 kDa
Poids moléculaire observé 22 kDa
Numéro d’acquisition GenBankBC050356
Symbole du gène RNF212
Identification du gène (NCBI) 285498
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNF212(RING finger protein 212)acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. It may be a SUMO E3 ligase that mediates the sumoylation of target proteins MSH4 and MSH5, leading to enhanced binding to recombination sites.

Protocole

Product Specific Protocols
WB protocol for RNF212 antibody 21178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

iru (Verified Customer) (07-14-2020)

I got a clean band but it is higher than the expected molecular weight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells
RNF212 Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:21178-1-AP)