Anticorps Polyclonal de lapin anti-RNF168

• Phare
• Validé par KD/KO

RNF168 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (2)

N° de cat : 21393-1-AP

Synonymes

ring finger protein 168, RNF168

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Galerie de données de validation

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  WB analysis of HepG2 using 21393-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21393-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 21393-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

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  IP experiment of HepG2 using 21393-1-AP

  IP result of anti-RNF168 (IP:21393-1-AP, 4ug; Detection:21393-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IF Staining of HepG2 using 21393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF168 antibody (21393-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293 transfectées, cellules HeLa, cellules Jurkat, tissu cérébral humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

21393-1-AP cible RNF168 dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNF168 Protéine recombinante Ag16055
• Nom complet: ring finger protein 168
• Masse moléculaire calculée: 571 aa, 65 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC033791
• Symbole du gène: RNF168
• Identification du gène (NCBI): 165918
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	IP	Nat Commun; Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.; Authors - Qinglei Hang
human		Nucleic Acids Res; GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.; Authors - Xiaopeng Lu
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB	EMBO Rep; An LTR retrotransposon-derived lncRNA interacts with RNF169 to promote homologous recombination.; Authors - Bing Deng
human	WB	FASEB J; RNF8-ubiquitinated KMT5A is required for RNF168-induced H2A ubiquitination in response to DNA damage.; Authors - Xiaopeng Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shunbin (Verified Customer) (05-14-2024)

Great. A single specific band, no background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

RNF168 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:21393-1-AP)

FH

Shunbin (Verified Customer) (05-14-2024)

Great. A single specific band without background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

RNF168 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:21393-1-AP)