Anticorps Polyclonal de lapin anti-RNASEH2C

• Phare
• Validé par KD/KO

RNASEH2C Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16518-1-AP

Synonymes

AGS3, AYP1, Ribonuclease H2 subunit C, ribonuclease H2, subunit C, Ribonuclease HI subunit C, RNase H1 small subunit, RNase H2 subunit C, RNASEH2C

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Galerie de données de validation

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  WB analysis using 16518-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16518-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 16518-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16518-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16518-1-AP (RNASEH2C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16518-1-AP

  IP result of anti-RNASEH2C (IP:16518-1-AP, 3ug; Detection:16518-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human brain using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16518-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16518-1-AP (RNASEH2C antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules Jurkat, tissu splénique humain, tissu testiculaire humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral humain, tissu cutané humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

16518-1-AP cible RNASEH2C dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNASEH2C Protéine recombinante Ag9739
• Nom complet: ribonuclease H2, subunit C
• Masse moléculaire calculée: 164 aa, 18 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC023588
• Symbole du gène: RNASEH2C
• Identification du gène (NCBI): 84153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nature; CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions.; Authors - Michal Zimmermann
human	WB	Hum Mol Genet; Reduction of hRNase H2 activity in Aicardi-Goutières Syndrome cells leads to replication stress and genome instability.; Authors - Sara Pizzi
human	WB	J Biol Chem; Analysis of ribonucleotide removal from DNA by human nucleotide excision repair.; Authors - Laura A Lindsey-Boltz
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li ; KD Validated