Anticorps Polyclonal de lapin anti-RNASEH2B

• Phare
• Validé par KD/KO

RNASEH2B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 17527-1-AP

Synonymes

AGS2, DLEU8, Ribonuclease H2 subunit B, ribonuclease H2, subunit B, Ribonuclease HI subunit B, RNase H2 subunit B, RNASEH2B

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Galerie de données de validation

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  WB analysis of mouse thymus using 17527-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17527-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17527-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17527-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17527-1-AP (RNASEH2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules HeLa, cellules MCF-7, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

17527-1-AP cible RNASEH2B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNASEH2B Protéine recombinante Ag11189
• Nom complet: ribonuclease H2, subunit B
• Masse moléculaire calculée: 331 aa, 37 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC036744
• Symbole du gène: RNASEH2B
• Identification du gène (NCBI): 79621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.

Publications

Species	Application	Title
rat	WB	Nutr Metab (Lond); Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model.; Authors - Shuo Cong
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li ; KD Validated