Anticorps Polyclonal de lapin anti-RNASEH2A

• Phare
• Validé par KD/KO

RNASEH2A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16132-1-AP

Synonymes

AGS4, JUNB, Ribonuclease H2 subunit A, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, RNase H(35), RNase H2 subunit A, RNase HI large subunit, RNASEH2A, RNASEHI, RNHIA, RNHL

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Galerie de données de validation

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  WB analysis using 16132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16132-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu hépatique humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Informations sur le produit

16132-1-AP cible RNASEH2A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RNASEH2A Protéine recombinante Ag9118
• Nom complet: ribonuclease H2, subunit A
• Masse moléculaire calculée: 299 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC011748
• Symbole du gène: RNASEH2A
• Identification du gène (NCBI): 10535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Publications

Species	Application	Title
human	WB	Commun Biol; RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells; Authors - Sho Sugawara
human	WB	PLoS Genet; Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.; Authors - Sarah K Deasy ; KD Validated
		Oncol Rep; RNaseH2A is involved in human gliomagenesis through the regulation of cell proliferation and apoptosis.; Authors - Bin Dai
human	IHC	Tumori; Hypoxia-induced RNASEH2A limits activation of cGAS-STING signaling in HCC and predicts poor prognosis.; Authors - Fengbo Zhao
human	WB,IHC	Cancer Res Commun; Ribonuclease H2 Subunit A Preserves Genomic Integrity and Promotes Prostate Cancer Progression; Authors - Naoki Kimura ; KD Validated
human,mouse	WB	bioRxiv; RTF2 controls replication repriming and ribonucleotide excision at the replisome; Authors - Brooke A Conti