• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-RNASEH1

RNASEH1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

N° de cat : 15606-1-AP

Synonymes

H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1



Applications testées

Résultats positifs en WBcellules HeLa, cellules U2OS
Résultats positifs en IPcellules HeLa,
Résultats positifs en IF/ICCcellules HeLa,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15606-1-AP cible RNASEH1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RNASEH1 Protéine recombinante Ag7990
Nom complet ribonuclease H1
Masse moléculaire calculée 32 kDa
Poids moléculaire observé 32 kDa
Numéro d’acquisition GenBankBC002973
Symbole du gène RNASEH1
Identification du gène (NCBI) 246243
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Protocole

Product Specific Protocols
WB protocol for RNASEH1 antibody 15606-1-APDownload protocol
IF protocol for RNASEH1 antibody 15606-1-APDownload protocol
IP protocol for RNASEH1 antibody 15606-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma.

Authors - Aparna Gorthi
humanWB

Cell

Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy.

Authors - Luciano E Marasco
humanWB

Nature

BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.

Authors - Vaibhav Bhatia
  • KD Validated
humanWB

Cell Res

Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells.

Authors - Junyu Wu
mouseIF

Nat Cell Biol

Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks.

Authors - Flavia Michelini
humanWB

Mol Cell

METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation.

Authors - Canfeng Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

R (Verified Customer) (06-15-2024)

Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
FH

Andrea (Verified Customer) (10-05-2023)

Strong signal, very good detection.

  • Applications: Western Blot
FH

Huai-Chin (Verified Customer) (06-08-2019)

Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:3000 for Western, 1:500 for IHC, 1:200 for IF-IC
  • Cell Tissue Type: Mammary gland/ MCF7