- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-RNASEH1
RNASEH1 Polyclonal Antibody for WB, IP, IF, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF/ICC, IP, ChIP, ELISA
Conjugaison
Non conjugué
N° de cat : 15606-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules U2OS |
Résultats positifs en IP | cellules HeLa, |
Résultats positifs en IF/ICC | cellules HeLa, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 4 publications below |
WB | See 30 publications below |
IHC | See 1 publications below |
IF | See 17 publications below |
ChIP | See 1 publications below |
Informations sur le produit
15606-1-AP cible RNASEH1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | RNASEH1 Protéine recombinante Ag7990 |
Nom complet | ribonuclease H1 |
Masse moléculaire calculée | 32 kDa |
Poids moléculaire observé | 32 kDa |
Numéro d’acquisition GenBank | BC002973 |
Symbole du gène | RNASEH1 |
Identification du gène (NCBI) | 246243 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].
Protocole
Product Specific Protocols | |
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WB protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
IF protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
IP protocol for RNASEH1 antibody 15606-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature EWS-FLI1 increases transcription to cause R-loops and block BRCA1 repair in Ewing sarcoma. | ||
Cell Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy. | ||
Nature BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
| ||
Cell Res Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells. | ||
Nat Cell Biol Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaks. | ||
Mol Cell METTL3 and N6-Methyladenosine Promote Homologous Recombination-Mediated Repair of DSBs by Modulating DNA-RNA Hybrid Accumulation. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH R (Verified Customer) (06-15-2024) | Excellent antibody working on WB (1/1000) and Immunofluorescence (1/250) with HeLa cells
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FH Andrea (Verified Customer) (10-05-2023) | Strong signal, very good detection.
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FH Huai-Chin (Verified Customer) (06-08-2019) | Very good antibody. I've tried it in Western blot, cultured cell immunofluorescence, and tissue IHC. All works well.
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