• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-RLIM

RLIM Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 16121-1-AP

Synonymes

NY REN 43, R LIM, RING finger protein 12, RLIM, RNF12



Applications testées

Résultats positifs en WBcellules HeLa, cellules Jurkat
Résultats positifs en IPcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

16121-1-AP cible RLIM dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RLIM Protéine recombinante Ag9077
Nom complet ring finger protein, LIM domain interacting
Masse moléculaire calculée 624 aa, 69 kDa
Poids moléculaire observé 75 kDa
Numéro d’acquisition GenBankBC013357
Symbole du gène RLIM
Identification du gène (NCBI) 51132
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RLIM (RING finger LIM domain-binding protein), also known as RNF12 (RING finger protein 12) or NY-REN-43, is a 624 amino acid RING-H2 zinc finger protein that is involved in protein ubiquitinylation and subsequent degradation. Expressed in a variety of tissues, RLIM binds to the LIM domain of various proteins and functions as a protein ligase that negatively co-regulates LIM homeodomain (LIM-HD) transcription factors. Through its interaction with Sin3A, a component of the histone deacetylase corepressor complex, RLIM is able to recruit the corepressor complex to LIM-HD proteins, thereby inhibiting LIM-HD transcription. In addition to recruiting the deacetylase complex to LIM-HD proteins, RLIM is able to bind to, ubiquinate and subsequently degrade CLIM proteins, which function as positive co-regulators of LIM-HD transcription factors. RLIM contains one RING-type zinc finger and is implicated in renal cell carcinoma. The calcualted molecular weight of RLIM is 69 kDa, but modified RLIM is about 70-75 kDa.

Protocole

Product Specific Protocols
WB protocol for RLIM antibody 16121-1-APDownload protocol
IP protocol for RLIM antibody 16121-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Cell Death Dis

RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis.

Authors - Yongsheng Huang
mouseWB

Elife

MOF-associated complexes ensure stem cell identity and Xist repression.

Authors - Tomasz Chelmicki
humanWB

Oncotarget

PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.

Authors - Chao Li
human,mouseWB,IHC,IF,CoIP

J Mol Cell Biol

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Authors - Yuwei Li
  • KD Validated
humanWB

Oncotarget

RLIM suppresses hepatocellular carcinogenesis by up-regulating p15 and p21.

Authors - Yongsheng Huang