- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-RLIM
RLIM Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 16121-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellules Jurkat |
Résultats positifs en IP | cellules HeLa |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Informations sur le produit
16121-1-AP cible RLIM dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | RLIM Protéine recombinante Ag9077 |
Nom complet | ring finger protein, LIM domain interacting |
Masse moléculaire calculée | 624 aa, 69 kDa |
Poids moléculaire observé | 75 kDa |
Numéro d’acquisition GenBank | BC013357 |
Symbole du gène | RLIM |
Identification du gène (NCBI) | 51132 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
RLIM (RING finger LIM domain-binding protein), also known as RNF12 (RING finger protein 12) or NY-REN-43, is a 624 amino acid RING-H2 zinc finger protein that is involved in protein ubiquitinylation and subsequent degradation. Expressed in a variety of tissues, RLIM binds to the LIM domain of various proteins and functions as a protein ligase that negatively co-regulates LIM homeodomain (LIM-HD) transcription factors. Through its interaction with Sin3A, a component of the histone deacetylase corepressor complex, RLIM is able to recruit the corepressor complex to LIM-HD proteins, thereby inhibiting LIM-HD transcription. In addition to recruiting the deacetylase complex to LIM-HD proteins, RLIM is able to bind to, ubiquinate and subsequently degrade CLIM proteins, which function as positive co-regulators of LIM-HD transcription factors. RLIM contains one RING-type zinc finger and is implicated in renal cell carcinoma. The calcualted molecular weight of RLIM is 69 kDa, but modified RLIM is about 70-75 kDa.
Protocole
Product Specific Protocols | |
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WB protocol for RLIM antibody 16121-1-AP | Download protocol |
IP protocol for RLIM antibody 16121-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Dis RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis. | ||
Oncotarget PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1. | ||
J Mol Cell Biol Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.
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