Anticorps Polyclonal de lapin anti-RLBP1

RLBP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 15356-1-AP

Synonymes

CRALBP, RLBP1

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Galerie de données de validation

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  WB analysis using 15356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15356-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15356-1-AP (RLBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded human eye using 15356-1-AP (RLBP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 15356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 15356-1-AP (RLBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Retinal organoids using 15356-1-AP

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Tubulin beta 3/TUJ1 using 66375-1-Ig at 1:500 dilution (green) and CRALBP using 15356-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 50 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

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  IF Staining of mouse eye using 15356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 15356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (15356-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris, cellules HepG2, tissu oculaire de rat, tissu rétinien de souris
• Résultats positifs en IHC: tissu oculaire de souris, tissu oculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu oculaire de souris, Retinal organoids

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Informations sur le produit

15356-1-AP cible RLBP1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RLBP1 Protéine recombinante Ag7602
• Nom complet: retinaldehyde binding protein 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC004199
• Symbole du gène: RLBP1
• Identification du gène (NCBI): 6017
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).

Publications

Species	Application	Title
zebrafish	IF	Aging Cell; Müller Glia maintain their regenerative potential despite degeneration in the aged zebrafish retina.; Authors - Raquel R Martins
human	WB,IF	Mol Vis; Morphological, biochemical, and transcriptomic characterization of iPSC-derived human RPE cells from normal and Smith-Lemli-Opitz syndrome patients; Authors - Michael H Farkas
mouse	IF	Diabetes; CD40 in Retinal Müller Cells Induces P2X7-Dependent Cytokine Expression in Macrophages/Microglia in Diabetic Mice and Development of Early Experimental Diabetic Retinopathy.; Authors - Jose-Andres C Portillo
mouse	WB	FASEB J; A cell-penetrating CD40-TRAF2,3 blocking peptide diminishes inflammation and neuronal loss after ischemia/reperfusion.; Authors - Jose-Andres C Portillo
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
human	IF	Invest Ophthalmol Vis Sci; CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression; Authors - Sarah Vos

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval in NaCit 0.1M ph 6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Retina

RLBP1 Antibody Immunohistochemistry, validation (1:100 dilution) in Retina (Cat no:15356-1-AP)