- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-RIPK2
RIPK2 Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IP, IF, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 15366-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HEK-293T, cellules HeLa, cellules THP-1, tissu cérébral de souris |
Résultats positifs en IP | cellules HEK-293T, |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 10 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Informations sur le produit
15366-1-AP cible RIPK2 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, porc, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | RIPK2 Protéine recombinante Ag0909 |
Nom complet | receptor-interacting serine-threonine kinase 2 |
Masse moléculaire calculée | 61 kDa |
Poids moléculaire observé | 46-55 kDa, 61-72 kDa |
Numéro d’acquisition GenBank | BC004553 |
Symbole du gène | RIPK2 |
Identification du gène (NCBI) | 8767 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.
Protocole
Product Specific Protocols | |
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WB protocol for RIPK2 antibody 15366-1-AP | Download protocol |
IP protocol for RIPK2 antibody 15366-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Antioxidants (Basel) M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide | ||
Obesity (Silver Spring) Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance. | ||
J Pharmacol Exp Ther Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.
| ||
Front Cell Infect Microbiol Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways. | ||
Immunol Lett A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation. | ||
Front Genet Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.
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