• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-RIPK2

RIPK2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 15366-1-AP

Synonymes

CARD3, CARDIAK, CCK, GIG30, Receptor interacting protein 2, RICK, RIP 2, RIP2, RIPK2



Applications testées

Résultats positifs en WBcellules HEK-293T, cellules HeLa, cellules THP-1, tissu cérébral de souris
Résultats positifs en IPcellules HEK-293T,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15366-1-AP cible RIPK2 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéeHumain, porc, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RIPK2 Protéine recombinante Ag0909
Nom complet receptor-interacting serine-threonine kinase 2
Masse moléculaire calculée 61 kDa
Poids moléculaire observé 46-55 kDa, 61-72 kDa
Numéro d’acquisition GenBankBC004553
Symbole du gène RIPK2
Identification du gène (NCBI) 8767
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK2 is also named as CARDIAK, RICK, RIP2 and belongs to the receptor-interacting serine/threonine-protein kinase(RIPK) family. RIPK2 functions as a key signaling protein in host defense responses induced by activation of the cytosolic pattern recognition receptors (PRR) NOD1 and NOD2. It has also been reported to mediate other cellular responses, including TLR signaling, caspase 1 activation , apoptosis and autophagy(PMID:22685397). It has 2 isoforms produced by alternative splicing.

Protocole

Product Specific Protocols
WB protocol for RIPK2 antibody 15366-1-APDownload protocol
IP protocol for RIPK2 antibody 15366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

Antioxidants (Basel)

M6A RNA Methylation Mediates NOD1/NF-kB Signaling Activation in the Liver of Piglets Challenged with Lipopolysaccharide

Authors - Menghui Xu
humanWB

Obesity (Silver Spring)

Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.

Authors - Yanping Wang
humanWB

J Pharmacol Exp Ther

Identification and Characterization of Novel Receptor-Interacting Serine/Threonine-Protein Kinase 2 Inhibitors Using Structural Similarity Analysis.

Authors - Mohamed Salla
  • KD Validated
mouseWB

Front Cell Infect Microbiol

Extracellular Histone H3 Induces Pyroptosis During Sepsis and May Act Through NOD2 and VSIG4/NLRP3 Pathways.

Authors - Chun-Xia Shi
mouseWB,IP

Immunol Lett

A small molecule inhibitor of caspase-1 inhibits NLRP3 inflammasome activation and pyroptosis to alleviate gouty inflammation.

Authors - Dong-Yi Cao
humanWB

Front Genet

Knockdown of RIPK2 Inhibits Proliferation and Migration, and Induces Apoptosis via the NF-κB Signaling Pathway in Gastric Cancer.

Authors - Qian Yang
  • KD Validated