Anticorps Polyclonal de lapin anti-MIRO2

• Phare
• Validé par KD/KO

MIRO2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

22

Avis (1)

N° de cat : 11237-1-AP

Synonymes

ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2

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Galerie de données de validation

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  WB analysis using 11237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11237-1-AP (MIRO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11237-1-AP

  WB result of MIRO2 antibody (11237-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MIRO2 transfected HepG2 cells.

• 

  WB analysis using 11237-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11237-1-AP (MIRO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11237-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11237-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11237-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11237-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11237-1-AP (RHOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11237-1-AP

  IP result of anti-MIRO2 (IP:11237-1-AP, 4ug; Detection:11237-1-AP 1:4000) with L02 cells lysate 3080 ug.

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  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11237-1-AP (MIRO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11237-1-AP (RHOT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11237-1-AP (RHOT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11237-1-AP (RHOT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11237-1-AP (RHOT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11237-1-AP (RHOT2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11237-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11237-1-AP (RHOT2 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules K-562, cellules L02, tissu cardiaque de souris, tissu cardiaque humain, tissu rénal humain, tissu testiculaire humain
• Résultats positifs en IP: cellules L02,
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain, tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below

Informations sur le produit

11237-1-AP cible MIRO2 dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIRO2 Protéine recombinante Ag1752
• Nom complet: ras homolog gene family, member T2
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC014942
• Symbole du gène: RHOT2
• Identification du gène (NCBI): 89941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle. MIRO2 is highly phosphorylated at basal conditions, as evidenced by an immunoreactive band that electrophoretically migrates 7 kDa higher (~75 kDa) compared to its predicted molecular weight (68 kDa) (PMID: 28556983).

Publications

Species	Application	Title
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
		Neuron; Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.; Authors - Juliet Goldsmith
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
human	IF	EMBO Rep; Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes.; Authors - Fumika Koyano
human	WB, IP, IF	EMBO Rep; MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.; Authors - Fei Wang
human	WB, IF	Mol Ther Nucleic Acids; miR-351-5p/Miro2 axis contributes to hippocampal neural progenitor cell death via unbalanced mitochondrial fission.; Authors - Ha-Na Woo ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tim (Verified Customer) (11-25-2021)

Tested on HeLa total cell lysate. Very good.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

MIRO2 Antibody Western Blot validation (1/1000 dilution) in HeLa (Cat no:11237-1-AP)