Anticorps Polyclonal de lapin anti-RHOG-Specific

RHOG-Specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 19671-1-AP

Synonymes

ARHG, RHOG, RHOG-Specific

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 19671-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19671-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19671-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 19671-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 19671-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 19671-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19671-1-AP (RHOG-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 19671-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 19671-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19671-1-AP (RHOG-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s, cellules C6, cellules HeLa, cellules MCF-7, cellules Neuro-2a, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19671-1-AP cible RHOG-Specific dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ras homolog gene family, member G (rho G)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: NM_001665
• Symbole du gène: RHOG
• Identification du gène (NCBI): 391
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.