Anticorps Polyclonal de lapin anti-RHOG

RHOG Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 21576-1-AP

Synonymes

ARHG, RHOG

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Galerie de données de validation

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  WB analysis using 21576-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 21576-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21576-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21576-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21576-1-AP cible RHOG dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHOG Protéine recombinante Ag16118
• Nom complet: ras homolog gene family, member G (rho G)
• Masse moléculaire calculée: 191 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC104178
• Symbole du gène: RHOG
• Identification du gène (NCBI): 391
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.