Anticorps Polyclonal de lapin anti-RHOC

RHOC Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 10632-1-AP

Synonymes

ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9

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Galerie de données de validation

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  WB analysis using 10632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10632-1-AP (RHOC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10632-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10632-1-AP (RHOC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 10632-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10632-1-AP (RHOC Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules HeLa, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 2 publications below

Informations sur le produit

10632-1-AP cible RHOC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHOC Protéine recombinante Ag0988
• Nom complet: ras homolog gene family, member C
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC007245
• Symbole du gène: RHOC
• Identification du gène (NCBI): 389
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

Publications

Species	Application	Title
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo
human	IHC	FASEB J; Cytotoxic necrotizing factor 1 promotes bladder cancer angiogenesis through activating RhoC.; Authors - Yaxiu Guo
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Front Mol Biosci; CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma.; Authors - Fan Feng
human	WB, IHC	Mol Carcinog; The role of the long non-coding RNA TDRG1 in epithelial ovarian carcinoma tumorigenesis and progression through miR-93/RhoC pathway.; Authors - Shuo Chen
human	WB	PLoS One; Overexpression of RNF146 in Non-Small Cell Lung Cancer Enhances Proliferation and Invasion of Tumors through the Wnt/β-catenin Signaling Pathway.; Authors - Ying Gao