Anticorps Polyclonal de lapin anti-RGS9

RGS9 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17970-1-AP

Synonymes

PERRS, RGS9, RGS9L

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Galerie de données de validation

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  WB analysis of mouse brain using 17970-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17970-1-AP (RGS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17970-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17970-1-AP (RGS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17970-1-AP cible RGS9 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGS9 Protéine recombinante Ag11976
• Nom complet: regulator of G-protein signaling 9
• Masse moléculaire calculée: 622 aa, 71 kDa
• Poids moléculaire observé: 58 kDa, 70-75 kDa
• Numéro d’acquisition GenBank: BC022504
• Symbole du gène: RGS9
• Identification du gène (NCBI): 8787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RGS9 is a member of the RGS family of signaling proteins that suppress the activity of G proteins by promoting their deactivation. There are two splice isoforms of RGS9 with quite different properties and patterns of expression. RGS9-1 is mainly found in the eye and is involved in regulation of phototransduction in rod and cone cells of the retina, while RGS9-2 is is expressed specifically in the striatum, a brain region involved in controlling movement, motivation, mood and addition. This antibody got about 58kD in the mouse eye and 70kD in the mouse brain in western blotting, both in line with their expected masses. Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS).

Publications

Species	Application	Title
human	WB	Genes (Basel); Extended Phenotyping and Functional Validation Facilitate Diagnosis of a Complex Patient Harboring Genetic Variants in MCCC1 and GNB5 Causing Overlapping Phenotypes.; Authors - Zhuo Shao