Anticorps Polyclonal de lapin anti-RGMA

• Phare
• Validé par KD/KO

RGMA Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12387-1-AP

Synonymes

Repulsive guidance molecule A, RGM, RGM domain family member A, RGM domain family, member A, RGMA

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Galerie de données de validation

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  WB analysis using 12387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12387-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 12387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12387-1-AP

  IP result of anti-RGMA (IP:12387-1-AP, 4ug; Detection:12387-1-AP 1:400) with mouse testis tissue lysate 4000ug.

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  IHC staining of human brain using 12387-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12387-1-AP (RGMA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 12387-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12387-1-AP (RGMA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules Caco-2, cellules HeLa, cellules K-562, cellules MOLT-4, cellules U2OS, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu cérébral humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12387-1-AP cible RGMA dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGMA Protéine recombinante Ag3058
• Nom complet: RGM domain family, member A
• Masse moléculaire calculée: 450 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC015886
• Symbole du gène: RGMA
• Identification du gène (NCBI): 56963
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RGMA, also named as RGM, is a member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. It regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. RGMA binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. RGMA is a molecular target for neuroprotection in retinal pathologies(PMID: 20457227). the frequent genetic and epigenetic inactivation of RGMA in CRCs and adenomas along with its in vitro function collectively support its role as a tumor suppressor in colon cells(PMID:19303019 ). There're some different MW in WB detection, 30 kDa mature form, 47-50 kDa isoforms and 60 kDa Glycosylated form.

Publications

Species	Application	Title
rat	WB	Brain Res Bull; Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion.; Authors - Min Li ; KD Validated
mouse	CoIP	Sci Adv; RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS; Authors - Mikito Shimizu