Anticorps Polyclonal de lapin anti-COP1

COP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 13542-1-AP

Synonymes

COP1, hCOP1, RFWD2, RING finger protein 200, RNF200

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Galerie de données de validation

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  WB analysis of mouse heart using 13542-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13542-1-AP (COP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13542-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13542-1-AP (COP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human osteosarcoma using 13542-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 13542-1-AP (COP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 13542-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 13542-1-AP (COP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 13542-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 13542-1-AP (COP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human osteosarcoma using 13542-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 13542-1-AP (COP1 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu cardiaque de souris
• Résultats positifs en IHC: tissu d'ostéosarcome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

13542-1-AP cible COP1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COP1 Protéine recombinante Ag4484
• Nom complet: ring finger and WD repeat domain 2
• Masse moléculaire calculée: 731 aa, 80 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC039723
• Symbole du gène: COP1
• Identification du gène (NCBI): 64326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

COP1 is also named as RFWD2, RNF200 and belongs to the COP1 family. It is a ubiquitin ligase that targets key regulators for degradation, and DET1 complexes with COP10 and DDB1, which is proposed to aid in COP1-mediated degradation (PMID:22705257). The endogenous COP1 is localized predominantly in the nucleus, but small amount may also be present in the cytosol. Within the nucleus, COP1 is present in both the nucleoplasm (NP) and the nuclear envelope (NE) fractions, although COP1 is more enriched in the nucleoplasm (PMID:12466024). Two of the COP1 isoforms identified by RNA-seq analysis predicted the size of two proteins detected by the antibody for COP1, a 75 kDa band, corresponding to the longer isoform, and a 50 kDa band corresponding to the shorter isoform. (PMID:24714719). It has been shown that COP1 forms dimerization through its coiled-coil region.

Publications

Species	Application	Title
human	WB	Cancer Lett; Primate-specific miR-944 activates p53-dependent tumor suppression in human colorectal cancers.; Authors - Yoon-Jin Kim ; KD Validated