Anticorps Polyclonal de lapin anti-Renin receptor, ATP6AP2

Renin receptor, ATP6AP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10926-1-AP

Synonymes

ATP6AP2, Renin receptor, APT6M8 9, ATPase H(+)-transporting lysosomal accessory protein 2, ATP6M8-9

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Galerie de données de validation

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  WB analysis of human retinal pigment epithelium tissue using 10926-1-AP

  WB result from Oscar Alcazar, et al, (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling, Exp Eye Res. 89(5) 638-47 (2009) (PMID:19580809). Eye 45kd.

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  WB analysis using 10926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10926-1-AP (Renin receptor, ATP6AP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 10926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10926-1-AP (Renin receptor, ATP6AP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10926-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10926-1-AP (Renin receptor, ATP6AP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10926-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Renin receptor, ATP6AP2 antibody (10926-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu d'épithélium pigmentaire rétinien humain
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

10926-1-AP cible Renin receptor, ATP6AP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Renin receptor, ATP6AP2 Protéine recombinante Ag1360
• Nom complet: ATPase, H+ transporting, lysosomal accessory protein 2
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC010395
• Symbole du gène: Renin receptor
• Identification du gène (NCBI): 10159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP6AP2, also named as ATP6IP2, CAPER, ELDF10, N14F, ATP6M8-9, Renin receptor, and prorenin receptor, is believed to potentiate the renin-angiotensin system (RAS), conferring to prorenin, a likely pathological role at the tissue level. The PRR has been identified in the microvascular endothelial cells of the retina, which seems to be involved in pathological neovascularization processes. The present study demonstrates for the first time that the PRR is expressed in human ATP6AP2 and suggests a molecular mechanism by which hypertension may exacerbate the pathology of dry AMD. ATP6AP2 functions as a renin and prorenin cellular receptor. It may mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). Defects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE). The full length of ATP6AP2 protein is 39 kDa, and the band with an apparent molecular weight of 28 kDa is the soluble form.(PMID:19580809; PMID:28215051; PMID:34534267; PMID: 29127204)

Publications

Species	Application	Title
mouse	WB	J Clin Invest; mTORC1 feedback to AKT modulates lysosomal biogenesis through MiT/TFE regulation.; Authors - Kaushal Asrani
human	WB,IHC	Exp Eye Res; (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling.; Authors - Alcazar Oscar O
human	WB	Int J Clin Exp Pathol; MicroRNA-140-3p inhibits proliferation, migration and invasion of lung cancer cells by targeting ATP6AP2.; Authors - Xiao-Mei Kong
human	WB,IHC	Cell Biosci; PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression; Authors - Liang Chen
mouse	WB,IHC	Placenta; Endothelin-1 production via placental (pro)renin receptor in a mouse model of preeclampsia; Authors - Sakurako Mishima