Anticorps Polyclonal de lapin anti-Renin

Renin Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 14291-1-AP

Synonymes

Angiotensinogenase, REN, Renin

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Galerie de données de validation

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  WB analysis using 14291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14291-1-AP (Renin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14291-1-AP (Renin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:800 (under 10x lens).

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  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:800 (under 40x lens).

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  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14291-1-AP (Renin antibody at dilution of 1:200.

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  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 14291-1-AP (Renin antibody at dilution of 1:200.

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of mouse kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14291-1-AP (Renin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14291-1-AP (Renin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14291-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14291-1-AP (Renin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14291-1-AP

  Immunofluorescent analysis of HepG2 cells, using REN antibody 14291-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HepG2
• Résultats positifs en IHC: tissu rénal humain, tissu de gliome humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 11 publications below
• IF: See 4 publications below

Informations sur le produit

14291-1-AP cible Renin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Renin Protéine recombinante Ag5612
• Nom complet: renin
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC047752
• Symbole du gène: REN
• Identification du gène (NCBI): 5972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REN(Renin) is also named as angiotensinogenase and belongs to the peptidase A1 family. It is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Human prorenin and renin are synthesized in juxtaglomerular cells and it locates in the juxtaglomerular cells and afferent arteriole as cytoplasmic granules (PMID:19664745, 9453303). REN has 2 isoforms produced by alternative splicing with the molecular weight of 45-47kDa. The mature REN can be detected as 38-42 kDa due to the cleavage of signal peptide and propeptide, and also detected as 55-60 kDa with variable degrees of glycosylation (PMID: 20966072, 15226276).Defects in REN are a cause of renal tubular dysgenesis (RTD) and familial juvenile hyperuricemic nephropathy type 2 (HNFJ2).

Publications

Species	Application	Title
human	WB	Br J Pharmacol; Novel inhibitors of the cellular RAS components, Poricoic acids, target Smad3 phosphorylation and Wnt/β-catenin pathway against renal fibrosis.; Authors - Ming Wang
	WB	Plant Physiol; Lysine malonylation of DgnsLIPID TRANSFER PROTEIN1 at the K81 site improves cold resistance in chrysanthemum; Authors - Xiaoqin Liao
mouse	IHC	Hypertension; Dysregulation of Aldosterone Secretion in Mast Cell-Deficient Mice.; Authors - Hadrien-Gaël Boyer
rat	IHC	Antioxid Redox Signal; Silencing of central (Pro)renin receptor ameliorates salt-induced renal injury in CKD.; Authors - Jiawen Li
human	WB	J Agric Food Chem; Novel RAS Inhibitors Poricoic Acid ZG and Poricoic Acid ZH Attenuate Renal Fibrosis via a Wnt/β-Catenin Pathway and Targeted Phosphorylation of smad3 Signaling.; Authors - Ming Wang
human,mouse	WB,IF	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu