Anticorps Polyclonal de lapin anti-RECQL4

• Phare
• Validé par KD/KO

RECQL4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 17008-1-AP

Synonymes

ATP dependent DNA helicase Q4, DNA helicase, RecQ like type 4, RecQ protein like 4, RECQ4, RECQL4, RTS

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Galerie de données de validation

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  WB analysis using 17008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17008-1-AP

  IP result of anti-RECQL4 (IP:17008-1-AP, 4ug; Detection:17008-1-AP 1:1000) with HeLa cells lysate 1880 ug.

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  IHC staining of human gliomas using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 17008-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17008-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RECQL4 antibody (17008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de gliome humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

17008-1-AP cible RECQL4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RECQL4 Protéine recombinante Ag10707
• Nom complet: RecQ protein-like 4
• Masse moléculaire calculée: 1208 aa, 133 kDa
• Poids moléculaire observé: 145-150 kDa
• Numéro d’acquisition GenBank: BC013277
• Symbole du gène: RECQL4
• Identification du gène (NCBI): 9401
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
human	IHC	Cancer Biol Med; RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.; Authors - Guosheng Lyu ; KD Validated
human	WB	FASEB J; UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair.; Authors - Qiuling Huang
human	WB	J Biol Chem; The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.; Authors - Jiewei Wang
human	IF	Sci Rep; PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.; Authors - Sebastian Veith
human	IF	bioRxiv; USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication; Authors - Hannah L Mackay