Anticorps Polyclonal de lapin anti-REC8

REC8 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 10793-1-AP

Synonymes

Cohesin Rec8p, HR21spB, REC8, REC8 homolog (yeast), REC8L1, Rec8p

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Galerie de données de validation

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  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10793-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10793-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10793-1-AP (REC8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Informations sur le produit

10793-1-AP cible REC8 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: REC8 Protéine recombinante Ag1250
• Nom complet: REC8 homolog (yeast)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC010887
• Symbole du gène: REC8
• Identification du gène (NCBI): 9985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).

Publications

Species	Application	Title
human	WB	Clin Cancer Res; Ectopic Expression of Cancer-Testis Antigens in Cutaneous T-cell Lymphoma Patients.; Authors - Ivan V Litvinov
mouse	IF	Cell Cycle; Knockout of cyclin B1 in granulosa cells causes female subfertility.; Authors - Jinmei Cheng
		Cell Cycle; Elevated intracellular pH appears in aged oocytes and causes oocyte aneuploidy associated with the loss of cohesion in mice.; Authors - Jin-Mei Cheng
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
human	IF	Int J Mol Sci; "Mitotic Slippage" and Extranuclear DNA in Cancer Chemoresistance: A Focus on Telomeres.; Authors - Kristine Salmina
human	WB	Exp Cell Res; The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells.; Authors - Erenpreisa Jekaterina J