Anticorps Polyclonal de lapin anti-FOX2/RBM9

• Phare
• Validé par KD/KO

FOX2/RBM9 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (3)

N° de cat : 12498-1-AP

Synonymes

FOX2, RBM9, HNRBP2, Hexaribonucleotide-binding protein 2, fxh

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Galerie de données de validation

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  WB analysis using 12498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12498-1-AP

  WB result of FOX2/RBM9 antibody (12498-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOX2/RBM9 transfected HEK-293 cells.

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  WB analysis using 12498-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12498-1-AP

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with HepG2 cells lysate 2680 ug.

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  IP experiment of K-562 using 12498-1-AP

  IP result of anti-FOX2/RBM9 (IP:12498-1-AP, 4ug; Detection:12498-1-AP 1:1000) with K-562 cells lysate 1760 ug.

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  IHC staining of human breast cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12498-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12498-1-AP (FOX2/RBM9 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12498-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FOX2/RBM9 antibody (12498-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NCCIT, cellules Neuro-2a
• Résultats positifs en IP: cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral de souris, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12498-1-AP cible FOX2/RBM9 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: FOX2/RBM9 Protéine recombinante Ag3168
• Nom complet: RNA binding motif protein 9
• Masse moléculaire calculée: 390 aa, 41 kDa
• Poids moléculaire observé: 50 and 60 kDa
• Numéro d’acquisition GenBank: BC013115
• Symbole du gène: RBM9
• Identification du gène (NCBI): 23543
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

Publications

Species	Application	Title
human	WB,IF,CoIP	Nat Cell Biol; RBFOX2 recognizes N6-methyladenosine to suppress transcription and block myeloid leukaemia differentiation; Authors - Xiaoyang Dou
human	WB	iScience; Hypoxia induces hepatocellular carcinoma metastasis via the HIF-1α/METTL16/lnc-CSMD1-7/RBFOX2 axis; Authors - Yingchao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gaurav (Verified Customer) (12-19-2023)

Excellent antibody without non-specific bands. Works perfectly as described.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: A7R5 Smooth Muscle Cells

FOX2/RBM9 Antibody Western Blot validation (1:4000 dilution) in A7R5 Smooth Muscle Cells (Cat no:12498-1-AP)

FH

Gaurav (Verified Customer) (10-04-2023)

Excellent antibody for endogenous proteins and works very fine. The quality of results is up to the expectations in co-immunoprecipitation and western blotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: A7R5 smooth muscle cells (Rat)

FOX2/RBM9 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in A7R5 smooth muscle cells (Rat) (Cat no:12498-1-AP)

FH

Gaurav (Verified Customer) (10-04-2023)

Excellent antibody for endogenous proteins and works very fine. The quality of results is up to the expectations in co-immunoprecipitation and western blotting.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: A7R5 smooth muscle cells (Rat)

FOX2/RBM9 Antibody Western Blot,Immunoprecipitation validation (1:3000 dilution) in A7R5 smooth muscle cells (Rat) (Cat no:12498-1-AP)