Anticorps Polyclonal de lapin anti-RBM5

RBM5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19930-1-AP

Synonymes

G15, H37, LUCA15, Protein G15, RBM5, RMB5, RNA binding motif protein 5, RNA binding protein 5

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Galerie de données de validation

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  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19930-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19930-1-AP (RBM5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

19930-1-AP cible RBM5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM5 Protéine recombinante Ag13908
• Nom complet: RNA binding motif protein 5
• Masse moléculaire calculée: 815 aa, 92 kDa
• Poids moléculaire observé: 110-115 kDa
• Numéro d’acquisition GenBank: BC002957
• Symbole du gène: RBM5
• Identification du gène (NCBI): 10181
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM5, also named as RNA-binding protein 5, is a 815 amino acid protein, which contains 2 RRM (RNA recognition motif) domains and Belongs to the RBM5/RBM10 family. RBM5 localizes in the nucleus and is a component of the spliceosome A complex. RBM5 regulates alternative splicing of a number of mRNAs and may modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. RBM5 may both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. The phosphorylation of RBM5 protein would influence the detected molecular weight. A 105-110 KDa band was also reported (PMID: 27957556).

Publications

Species	Application	Title
human	WB	J Biol Chem; CHIP suppresses the proliferation and migration of A549 cells by mediating the ubiquitination of eIF2α and upregulation of tumor suppressor RBM5; Authors - Bo Jin
mouse	WB	J Neurotrauma; RNA Binding Motif 5 Gene Deletion Modulates Cell Signaling in a Sex-Dependent Manner but not Hippocampal Cell Death.; Authors - Jeffrey Farooq
mouse	WB	Neuroscience; Identification of Novel Targets of RBM5 in the Healthy and Injured Brain.; Authors - Travis C Jackson
mouse	WB	Cell Death Discov; Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner; Authors - Kara Snyder