Anticorps Polyclonal de lapin anti-RBM39

• Phare
• Validé par KD/KO

RBM39 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, RIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 21339-1-AP

Synonymes

CAPER, CAPERalpha, CC1.3, fSAP59, HCC1, RBM39, RNA binding motif protein 39, RNA binding protein 39, RNPC2, Splicing factor HCC1

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Galerie de données de validation

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  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21339-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21339-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 21339-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 21339-1-AP (RBM39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 21339-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21339-1-AP (RBM39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 21339-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 21339-1-AP (RBM39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 21339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21339-1-AP (RBM39 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, tissu de muscle squelettique humain, tissu rénal de souris
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

21339-1-AP cible RBM39 dans les applications de WB, IHC, IF/ICC, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM39 Protéine recombinante Ag15584
• Nom complet: RNA binding motif protein 39
• Masse moléculaire calculée: 530 aa, 59 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC141835
• Symbole du gène: RBM39
• Identification du gène (NCBI): 9584
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM39, also named as HCC1 or RNPC2, is a 530 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the splicing factor SR family. RBM39 is widely expressed in various tissues and with highly expression in pancreas, skeletal muscle, lung and brain. RBM39 is a transcriptional coactivator for steroid nuclear receptors ESR1/ER-alpha and ESR2/ER-beta, and JUN/AP-1. RBM39 may be involved in pre-mRNA splicing process.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	J Proteome Res; Proximity Labeling, Quantitative Proteomics, and Biochemical Studies Revealed the Molecular Mechanism for the Inhibitory Effect of Indisulam on the Proliferation of Gastric Cancer Cells.; Authors - Jiaqi Lu ; KD Validated
human	WB	Cell Rep; Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.; Authors - Tabitha C Ting
human	IHC,RIP	Cell Death Discov; The cancer-testis lncRNA LINC01977 promotes HCC progression by interacting with RBM39 to prevent Notch2 ubiquitination; Authors - Anliang Xia
human	WB	Mol Cell Biochem; LncRNA MCM3AP-AS1 promotes chemoresistance in triple-negative breast cancer through the miR-524-5p/RBM39 axis; Authors - Yueping Wang