Anticorps Polyclonal de lapin anti-RBM16

RBM16 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 18893-1-AP

Synonymes

CCAP7, KIAA1116, RBM16, RNA binding motif protein 16, RNA binding protein 16, SCAF8

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Galerie de données de validation

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  WB analysis of HT-1080 using 18893-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18893-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18893-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 18893-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18893-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 18893-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18893-1-AP

  IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug.

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  IHC staining of human testis using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cervical cancer using 18893-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 18893-1-AP

  Immunofluorescent analysis of Hela cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 18893-1-AP

  Immunofluorescent analysis of HepG2 cells, using RBM16 antibody 18893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HT-1080, cellules L02, cellules NIH/3T3, cellules THP-1
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18893-1-AP cible RBM16 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM16 Protéine recombinante Ag13499
• Nom complet: RNA binding motif protein 16
• Masse moléculaire calculée: 1271 aa, 141 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC070071
• Symbole du gène: RBM16
• Identification du gène (NCBI): 22828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )