Anticorps Polyclonal de lapin anti-RBM14

RBM14 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10196-1-AP

Synonymes

COAA, Paraspeckle protein 2, PSP2, RBM14, RNA binding motif protein 14, RNA binding protein 14, SIP, SYT interacting protein, SYTIP1, TMEM137

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Galerie de données de validation

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  WB analysis of Jurkat using 10196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10196-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10196-1-AP (RBM14 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

10196-1-AP cible RBM14 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM14 Protéine recombinante Ag0254
• Nom complet: RNA binding motif protein 14
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC000488
• Symbole du gène: RBM14
• Identification du gène (NCBI): 10432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM14 is a ribonucleoprotein that functions as a general nuclear coactivator, and an RNA splicing modulator. This protein contains two RNA recognition motifs (RRM) at the N-terminus, and multiple hexapeptide repeat domain at the C-terminus that interacts with thyroid hormone receptor-binding protein (TRBP), and is required for transcription activation.

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol; TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.; Authors - Xinxin Zuo
human	WB	Exp Mol Med; The deubiquitinating enzyme STAMBP is a newly discovered driver of triple-negative breast cancer progression that maintains RAI14 protein stability; Authors - Qianqian Yang
human	WB	Brain Res; Immunoprecipitation and mass spectrometry defines an extensive RBM45 protein-protein interaction network.; Authors - Yang Li
pig	WB	J Virol; RBM14 inhibits the replication of porcine epidemic diarrhea virus by recruiting p62 to degrade nucleocapsid protein through the activation of autophagy and interferon pathway; Authors - Xiaoquan Wang