Anticorps Polyclonal de lapin anti-RARA

RARA Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 10331-1-AP

Synonymes

NR1B1, RAR, RAR alpha, RARA, Retinoic acid receptor alpha, retinoic acid receptor, alpha

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Galerie de données de validation

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  WB analysis of K-562 using 10331-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10331-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10331-1-AP (RARA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10331-1-AP

  IP result of anti-RARA (IP:10331-1-AP, 4ug; Detection:10331-1-AP 1:300) with HeLa cells lysate 2600 ug.

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10331-1-AP (RARA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10331-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10331-1-AP (RARA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10331-1-AP cible RARA dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RARA Protéine recombinante Ag0420
• Nom complet: retinoic acid receptor, alpha
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC008727
• Symbole du gène: RARA
• Identification du gène (NCBI): 5914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Br J Cancer; All-trans retinoic acid enhances the cytotoxic effect of decitabine on myelodysplastic syndromes and acute myeloid leukaemia by activating the RARα-Nrf2 complex; Authors - Lu Wang
mouse	WB,IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB	Front Cell Dev Biol; Silybin Prevents Prostate Cancer by Inhibited the ALDH1A1 Expression in the Retinol Metabolism Pathway.; Authors - Ying Jiang
human	WB	Cell Signal; ATRA-mediated-crosstalk between stellate cells and Kupffer cells inhibits autophagy and promotes NLRP3 activation in acute liver injury.; Authors - Zhen Yu
human	IF	Andrology; A novel SNP in HUWE1 promoter confers increased risk of NOA by affecting the RA/RARα pathway in Chinese individuals; Authors - Xudong Shan
human	WB,CoIP	Biomedicines; Histone H3 Acetylation Is Involved in Retinoid Acid-Induced Neural Differentiation through Increasing Mitochondrial Function; Authors - Yang Zhang